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Image Search Results
Journal: Scientific Reports
Article Title: PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia
doi: 10.1038/s41598-018-36143-3
Figure Lengend Snippet: The characterization of ASC-derived EVs, collected after cell stimulation with PDGF. ( a , b ) The representative transmission electron microscopy images of cEVs ( a ) and PDGF-EVs ( b ) negatively stained with NanoVan (black line = 500 nm); ( c , d ) the representative diagram of cEVs ( c ) or PDGF-EVs ( d ) according to NTA; ( e ) the diagram of CD63 and CD81 concentration in cEVs and PDGF-EVs according to ELISA, (mean ± SEM, *p < 0.05 vs. cEVs, n = 6); ( f ) the representative western blot images of CD63 and CD81 expression in ASC-derived EVs, 10 μg of protein per lane; ( g ) the representative image of FACS analysis of CD63 expression on cEV (left) and PDGF-EV (right); ( h ) the representative image of FACS analysis of CD81 expression on cEV (left) and PDGF-EV (right); ( i ) the representative image of FACS analysis of phosphatidylserine expression on cEV (left) and PDGF-EV (right).
Article Snippet: After washing with PBS, wells were stained with biotinylated antibodies against to
Techniques: Derivative Assay, Cell Stimulation, Transmission Assay, Electron Microscopy, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing